As part of the activities carried out during the AfterLIFE period of the Mycorestore project, the IRNASA-CSIC team conducted in vivo measurements of chlorophyll, flavonoids, anthocyanins, and the balanced nitrogen index in the leaves of trees in the demonstration plots of La Alamedilla, El Cubo de Don Sancho, Linares de Riofrío, and Valdelosa during June 2024. This was done to monitor the condition of the trees in both healthy and diseased plots.